A sucrose binding protein homologue from soybean exhibits GTP-binding activity that functions independently of sucrose transport activity
نویسندگان
چکیده
منابع مشابه
Electrogenic sucrose transport in developing soybean cotyledons.
Addition of sucrose to a solution bathing an excised developing soybean cotyledon causes a transient depolarization of the membrane potential, as measured using standard electrophysiological techniques. The magnitude of the depolarization is dependent on the concentration of both sucrose and protons in a manner which suggests carrier mediation; this process has an apparent K(m) for sucrose of a...
متن کاملEnergetics of sucrose transport into protoplasts from developing soybean cotyledons.
The accumulation of tetraphenylphosphonium (TPP(+)), 5,5'-dimethyl-oxazolidine-2,4-dione (DMO), and a micro pH electrode were used to measure membrane potential, intracellular and extracellular pH, respectively, upon the addition of exogenous sucrose to soybean cotyledon protoplasts. Addition of sucrose caused a specific and transient (a) depolarization of the membrane potential (measured by TP...
متن کاملA 62-kD sucrose binding protein is expressed and localized in tissues actively engaged in sucrose transport.
Sucrose transport from the apoplasm, across the plasma membrane, and into the symplast is critical for growth and development in most plant species. Phloem loading, the process of transporting sucrose against a concentration gradient into the phloem, is an essential first step in long-distance transport of sucrose and carbon partitioning. We report here that a soybean 62-kD sucrose binding prot...
متن کاملAnalysis of the transport activity of barley sucrose transporter HvSUT1.
Localization studies indicate that barley (Hordeum vulgare) sucrose transporter HvSUT1 functions in sucrose uptake into seeds during grain filling. To further understand the physiological function of HvSUT1, we have expressed the HvSUT1 cDNA in Xenopus laevis oocytes and analyzed the transport activity by two-electrode voltage clamping. Consistent with a H(+)-coupled transport mechanism, sucros...
متن کاملA soybean seed protein with carboxylate-binding activity.
The seed coat serves as a multifunctional organ with a role in protection and for the supply of nutrients to the embryo sac during development. The composition of the legume seed coat differs from other seed tissues in many ways including its protein composition. An abundant 24 kDa protein (SC24) has been purified and identified from soybean (Glycine max [L.] Merr) seed hulls. The corresponding...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2002
ISSN: 0014-2956
DOI: 10.1046/j.1432-1033.2002.03089.x